Skip to main content

UVB-induced GM-CSF production is suppressed by dexamethasone in HaCaT Cells

Buy Article:

$51.00 plus tax (Refund Policy)

Abstract:

Background:

Epidermal keratinocytes are important sources of a wide variety of cytokines that include the Granulocyte-macrophage Colony Stimulating Factor (GM-CSF). Glucocorticoids have been shown to inhibit the production of several cytokines. However, their effect on GM-CSF synthesis by keratinocytes is still unknown. Methods:

The effects of glucocorticoid on GM-CSF production by keratinocytes were evaluated using ELISA and RT-PCR analysis. Results:

GM-CSF secretion by HaCaT cells increased with increasing UVB exposure. Dexamethasone suppressed basal release of GM-CSF. In addition, it strongly inhibited both the UVB-mediated augmentation of GM-CSF protein production and mRNA expression. Lincomycin enhanced slightly the inhibitory effect of dexamethasone on GM-CSF synthesis, while lincomycin itself had no effect on GM-CSF secretion. Conclusion:

Results showed that dexamethasone suppressed basal release and UVB-induced production of GM-CSF by keratinocytes.

Keywords: GM-CSF; UVB; glucocorticoids

Document Type: Research Article

DOI: http://dx.doi.org/10.1034/j.1600-0781.2001.170303.x

Affiliations: Department of Dermatology and

Publication date: June 1, 2001

mksg/ppp/2001/00000017/00000003/art00003
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more