Skip to main content

Collagen loss in photoaged human skin is overestimated by histochemistry

Buy Article:

The full text article is temporarily unavailable.

We apologise for the inconvenience. Please try again later.

It is well known that photoaged skin is characterized by increases in dermal matrix components that include glycosaminoglycans, proteoglycans and masses of abnormal elastic fibers accompanied by substantial collagen loss. Histochemical staining of such tissue gives the impression of “massive” loss of collagen and its replacement by these other matrix components. Early biochemical studies have lent support to this notion with a reported decrease in total collagen of ∼45% compared to protected skin. More recent studies report considerably less, but varying, amounts of collagen loss. Rarely have the two approaches, histochemistry and biochemical analysis, been used in the same study to examine the same tissue. In this study, collagen loss was quantified biochemically in paired biopsies from sun-protected and sun-exposed arm skin of moderately photoaged female subjects (age 51–77 years). The values obtained were compared with histochemical and immunochemical findings. Quantitatively, collagen loss on a per mg protein basis was small compared to the histochemical appearance.

No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: biochemistry; collagen; elastosis; glycosaminoglycans; immunohistochemistry; photoaging

Document Type: Original Article

Affiliations: 1: Departments of Dermatology, University of Pennsylvania School of Medicine, Philadelphia, PA and 2: Mount Sinai School of Medicine, New York, NY, USA

Publication date: 2000-10-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more