Skip to main content

Epidermal stratification reduces the effects of UVB (but not UVA) on keratinocyte cytokine production and cytotoxicity

Buy Article:

$43.00 plus tax (Refund Policy)

Ultraviolet (UV) radiation induces cytokine release from cultured keratinocytes as well as from epidermis in vivo. The purpose of this study was to determine whether differentiation of cultured keratinocytes into stratified epithelium decreases the effects of UVA and UVB radiation on cytokine release. Interleukin‐1 (IL‐1)α, IL‐1β and tumor necrosis factor (TNF)‐α release from human keratinocytes and reconstituted human epidermis was measured after exposure to UVA or UVB radiation. Release of IL‐1α, IL‐1β, and TNF‐α was induced by both UVA and UVB radiation from both keratinocytes and reconstituted epidermis. Release of these cytokines was correlated with cytotoxicity. Keratinocyte cultures were far more sensitive to UVB radiation than reconstituted epidermis, in terms of both cytotoxicity and cytokine release. In contrast, epidermal stratification/differentiation had much less effect on the sensitivity to UVA radiation. We conclude that epidermal stratification and the formation of a stratum corneum provide protection against UVB radiation but have limited barrier effect against UVA radiation.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Document Type: Original Article

Affiliations: 1: Center for Cosmetic Toxicology, Institute of Pharmacological Sciences, University of Milan, Milan, Italy 2: Departments of Dermatology and Pathology, Bowman Gray School of Medicine, Wake Forest University, Winston Salem, NC, USA

Publication date: 1997-08-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more