GM‐CSF production by epithelial cell line: Upregulation by ultraviolet A
Abstract:It was demonstrated that UVB increases synthesis and expression of IL‐1α and GM‐CSF by keratinocytes. Upregulation of GM‐CSF by UVB is reported to be mediated by IL‐1α. However, regulation of IL‐1α and GM‐CSF by UVA is not well‐known. The purpose of the present study was to evaluate the effects of UVA on IL‐1α and GM‐CSF production. Here we used a competitive RT‐PCR for measuring cytokine gene expression in an epidermal cell line after UVA irradiation. IL‐1α and GM‐CSF mRNA did not show any change at 1 h and 6 h following exposure to UVA. After UVA irradiation, however, IL‐1α mRNA decreased and GM‐CSF mRNA increased at 24 h and the level of GM‐CSF in culture supernatant increased at 24 h and 48 h. Addition of antihuman IL‐1α neutralizing antibody to UVA irradiated cells did not prevent the increase of GM‐CSF mRNA expression. These results suggest that UVA radiation may induce GM‐CSF production through an IL‐1α independent pathway.
Document Type: Original Article
Publication date: 1997-08-01