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8‐Methoxypsoralen photoadduct formation in complementary oligonucleotides containing a cross‐linkable site

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Abstract:

The complete profile of 8‐methoxypsoralen photoadduct formation in complementary oligonucleotides (5′‐GAGTATGAG and 5′‐CATAC) has been determined. Equimolar solutions of the oligonucleotides were irradiated at 4°C in order to stabilize the mini‐double helix. Photomodified oligonucleotides were separated by reversed phase chromatography on a Vydac C4 column. Photoadduct formation favored the 5′TAT site in the 9mer over the 5′ATA site in the 5mer by a factor of two. Split‐dose studies showed that the monoadducts formed on GAGTATGAG were preferencially converted to cross‐links by an additional UVA exposure.

Document Type: Original Article

DOI: http://dx.doi.org/10.1111/j.1600-0781.1995.tb00147.x

Affiliations: Photobiology Laboratory, Department of Dermatology, Yale University, New Haven, Connecticut, USA

Publication date: June 1, 1995

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