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Clonal accumulation of T cells bearing Vβ6 T-cell receptor in chronic inflammatory periodontal disease

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Our previous study demonstrated that among the various T-cell receptor Vβ gene segments, T-cell receptor Vβ6 gene–bearing T cells are predominant in periodontitis lesions. To explore the clonality of T cells expressing Vβ6 T-cell receptor, which is determined by the heterogeneity of the complementarity-determining region 3(CDR3), Vβ6-Cβ polymerase chain reaction products derived from gingival tissue and peripheral blood of periodontitis patients were analyzed by single-strand conformation polymorphism and nucleotide sequence. Both techniques provided quite similar results in terms of the number of accumulated clones in which clonal accumulation of T-cell receptor Vβ6-bearing T cells was found in most periodontitis lesions but not in peripheral blood. From the analysis of deduced amino acid sequences, a possible conserved amino acid residue in the CDR3 region of the periodontitis lesion-derived sequences was found in most patients. This conserved amino acid motif however, was not identified. Furthermore, skewed Jβ gene segment usage was observed for expanded clones from periodontitis lesions. Our results suggest that T cells infiltrating periodontitis lesions are responding to a restricted number of antigens or epitopes.
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Keywords: RT-PCR-SSCP; TCR; Vβ6; periodontitis; sequencing

Document Type: Research Article

Publication date: 01 August 2000

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