Ribosomes and secretory granules in human mast cells: close associations demonstrated by staining with a chelating agent

Authors: Dvorak, A.M.; Morgan, E.S.

Source: Immunological Reviews, Volume 179, Number 1, 1 February 2001 , pp. 94-101(8)

Publisher: Wiley-Blackwell

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Abstract:

Summary:

The distribution of ribosomes was investigated in mature human mast cells with a chelation-based staining protocol known to bleach DNA-rich structures, leaving RNA-rich structures unbleached. With this method, electron-dense ribosomes were adjacent to, attached to, and within secretory granules, which were also bleached with the chelation method that we used. The finding of these ribosome-secretory granule relationships suggests that secretory granules in mature human mast cells may participate in RNA metabolism.

Document Type: Original article

Affiliations: 1: Departments of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA.

Publication date: 2001-02-01