IngentaConnect Effect of combined T- and B-cell depletion of allogeneic HLA-mism...

Authors: Daorong Liu¹; Charlotte Tammik²; Jie-Zhi Zou¹; Ingemar Ernberg¹; Maria G. Masucci¹; Olle Ringden²; Victor Levitsky¹

Source: Clinical Transplantation, Volume 18, Number 5, October 2004 , pp. 518-524(7)

Publisher: Blackwell Publishing

Abstract:

Liu D, Tammik C, Zou J-Z, Ernberg I, Masucci MG, Ringden O, Levitsky V. Effect of combined T- and B-cell depletion of allogeneic HLA-mismatched bone marrow graft on the magnitude and kinetics of Epstein–Barr virus load in the peripheral blood of bone marrow transplant recipients.

Clin Transplant 2004 DOI: 10.1111/j.1399-0012.2004.00198.x

Recipients of T-cell-depleted bone marrow (BM) transplants (BMT) frequently develop Epstein–Barr virus (EBV)-associated post-transplant lymphoproliferative disease (PTLD) preceded by a rapid and prominent increase of EBV load in the peripheral blood. The level of this increase positively correlates with the incidence of PTLD. Using a semiquantitative PCR assay we compared the blood levels of EBV-DNA in patients transplanted with either T-cell or T- and B-cell-depleted human leukocyte antigen (HLA)-mismatched BM grafts. Combined T- and B-cell depletion correlated with significantly lower maximal levels of EBV load, which were reached with slower kinetics. These data indicate that B-cell depletion of BM can be used for prophylaxis of PTLD in BM transplant recipients and can affect the long-term balance between EBV and its host.

Keywords: B-cell; Epstein–Barr virus; lymphoma

Document Type: Research article

DOI: 10.1111/j.1399-0012.2004.00198.x

Affiliations: 1: Microbiology and Tumor Biology Center 2: Department of Clinical Immunology and Center for Allogeneic Stem Cell Transplantation at Huddinge Hospital, Karolinska Institutet, Stockholm, Sweden

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