IngentaConnect PCR monitoring for tetracycline resistance genes in subgingival p...

Authors: Manch-Citron, Jean N.¹; Lopez, Gilbert H.²; Dey, Anjana¹; Rapley, John W.²; MacNeill, Simon R.²; Cobb, Charles M.²

Source: Journal of Clinical Periodontology, Volume 27, Number 6, June 2000 , pp. 437-446(10)

Publisher: Blackwell Publishing

Abstract:

Background:

The selection of antibiotic resistance genes during antibiotic therapy is a critical problem complicated by the transmission of resistance genes to previously sensitive strains via conjugative plasmids and transposons and by the transfer of resistance genes between gram-positive and gram-negative bacteria. The purpose of this investigation was to monitor the presence of selected tetracycline resistance genes in subgingival plaque during site specific tetracycline fiber therapy in 10 patients with adult periodontitis. Method:

The polymerase chain reaction (PCR) was used in separate tests for the presence of 3 tetracycline resistance genes (tetM, tetO and tetQ) in DNA purified from subgingival plaque samples. Samples were collected at baseline, i.e., immediately prior to treatment, and at 2 weeks, and 1, 3, and 6 months post-fiber placement. The baseline and 6-month samples were also subjected to DNA hybridization tests for the presence of 8 putative periodontal pathogenic bacteria. Results:

PCR analysis for the tetM resistance gene showed little or no change in 5 patients and a decrease in detectability in the remaining 5 patients over the 6 months following tetracycline fiber placement. The results for tetO and tetQ were variable showing either no change in detectability from baseline through the 6-month sampling interval or a slight increase in detectability over time in 4 of the 10 patients. DNA hybridization analysis showed reductions to unmeasurable levels of the putative periodontal pathogenic bacteria in all but 2 of the 10 patients. Conclusions:

These results complement earlier studies of tet resistance and demonstrate the efficacy of PCR monitoring for the appearance of specific resistance genes during and after antibiotic therapy.

Keywords: tetracycline resistance; tetracycline fiber therapy; adult periodontitis; polymerase chain reaction; tet resistance

Document Type: Research article

DOI: 10.1034/j.1600-051x.2000.027006437.x

Affiliations: 1: Oral Biology and 2: Periodontics, School of Dentistry, University of Missouri-Kansas City, USA

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PCR monitoring for tetracycline resistance genes in subgingival plaque following site-specific periodontal therapy: A preliminary report