Helicobacter pylori clarithromycin resistance detected by
Etest and TaqMan real‐time polymerase chain reaction: a comparative study
The aim of the work was to compare H. pylori clarithromycin‐resistance according two methods. Etest was performed on H. pylori isolated from gastric biopsy samples.
TaqMan Real‐Time‐ PCR (RT‐PCR) was
performed on paraffin‐embedded gastric biopsy samples of the same patients. Forty‐seven out of 88 strains were resistant to clarithromycin by Etest, whereas RT‐ PCR detected this resistance on paraffin‐embedded
specimens of 50 patients. RT‐ PCR performed on paraffin‐embedded biopsy specimens of 47 patients infected with H. pylori resistant to clarithromycin as detected by Etest, revealed the presence of a resistant
strain only in 40 samples. RT‐ PCR performed on samples of 41 patients harbouring clarithromycin‐susceptible H. pylori strains showed the presence of 31 susceptible and 10 resistant strains. RT‐ PCR
detected 18 cases with heteroresistant status. The difference between the two tests in detecting clarithromycin‐resistance was not statistically significant even if RT‐ PCR detected more resistant cases. The genotyping resistance on paraffin‐embedded
gastric biopsy specimens may be used to establish resistance to clarithromycin before the treatment when culture and susceptibility testing are not available. In case of failure of an empirical clarithromycin‐based triple antimicrobial treatment, RT‐ PCR
performed on paraffin‐embedded biopsy sample will establish the primary resistance to clarithromycin. In addition, this test can be useful for epidemiological investigation as well as for monitoring the evolution of clarithromycin resistance along the time.
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Document Type: Research Article
Publication date: 2012-09-01