Skip to main content

The cell envelope‐associated protein, LytR, regulates the cysteine protease SpeB in Streptococcus pyogenes

Buy Article:

$48.00 plus tax (Refund Policy)

Abstract:

Minami M, Ichikawa M, Ohta M, Hasegawa T. The cell envelope‐associated protein, LytR, regulates the cysteine protease SpeB in Streptococcus pyogenes. APMIS 2012; 120: 417–26.

The LytR family of cell envelope‐associated transcriptional attenuators in bacteria has been brought into focus of scientific interest on the expression of various virulence factors, as well as bacterial cell envelope maintenance. However, this protein of Streptococcus pyogenes has been only described as cell surface‐associated protein, and its function is completely unknown. We created lytR mutant strains from two independent S. pyogenes strains to analyze the function of LytR. The protease assay in culture supernatant showed that lytR mutant had the higher cysteine protease activity than wild‐type. Two‐dimensional gel electrophoresis and western blotting analysis revealed that the amount of cysteine protease, SpeB in lytR mutant was more compared with that in wild‐type. The level of speB mRNA in lytR mutant also increased compared with that of wild‐type. The membrane integrity and potential in lytR mutant also were decreased compared with that of wild‐type. Murine infection model showed that less survival was detected in mice inoculated with lytR mutant than that with wild‐type, and the size of wound lesion of mice with lytR mutant was larger than that with wild‐type. Our data suggest that the lytR regulates the expression of SpeB in S. pyogenes with relation to membrane integrity.

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2011.02847.x

Affiliations: 1: Department of Bacteriology, Nagoya City University Graduate School of Medical Sciences 2: Department of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya, Japan

Publication date: May 1, 2012

mksg/apm/2012/00000120/00000005/art00009
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more