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/human gingival fibroblasts co‐culture: the best natural association in answer to the 2‐hydroxyethyl methacrylate release

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Di Giulio M, D’Ercole S, Zara S, Cataldi A, Cellini L. Streptococcus mitis/human gingival fibroblasts co‐culture: the best natural association in answer to the 2‐hydroxyethyl methacrylate release. APMIS 2011.

One of the major components of dental polymerized resin‐based restorative materials is 2‐hydroxyethyl methacrylate (HEMA) and its release in monomeric form interferes with the oral cavity environment. This study aimed to evaluate HEMA monomeric effects on the co‐culture of Streptococcus mitis and human gingival fibroblasts (HGFs). Streptococcus mitis DS12 and S. mitis ATCC 6249 were co‐cultivated with HGF in the presence of HEMA (3 mM), for 48 and 72 h; the amount of sessile and planktonic cells, as well as the prokaryotic and eukaryotic cell viability were analyzed in treated and untreated samples. The treatment of S. mitis/HGFs with HEMA did not produce significant effects on the bacterial adhesion and induced an increase in planktonic S. mitis ATCC 6249 population after 48 and 72 h. HEMA increased significantly the planktonic S. mitis ATCC 6249 viability when co‐cultured with HGFs, while a cytotoxic effect on HGFs, without bacteria, was recorded. An increase of bacterial aggregation on HGFs was also detected with HEMA. Data obtained in this study suggest that HEMA exhibits a toxic effect mainly on eukaryotic cells and this effect can be modulated by co‐cultivation with the S. mitis cells which, in the presence of the monomer, enhance their aggregation rate on HGFs.
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Document Type: Research Article

Affiliations: 1: Departments of Drug Sciences 2: Biomedical Sciences 3: Medicine and Ageing Sciences, University ‘G. d’Annunzio’, Chieti-Pescara, Italy

Publication date: 01 February 2012

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