Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry identification of
Abstract:Dridi B, Raoult D, Drancourt M. Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry identification of Archaea: towards the universal identification of living organisms. APMIS 2012; 120: 85–91.
Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS) identification of Archaea has been limited to some environmental extremophiles belonging to distant taxa. We developed a specific protocol for MALDI‐TOF‐MS identification of Archaea and applied it to seven environmental human‐associated Methanobrevibacter smithii, Methanobrevibacter oralis, Methanosphaera stadtmanae, and the recently described Methanomassiliicoccus luminyensi Archaea. After mechanical lyse, we observed a unique protein profile for each organisms comprising 7–24 peaks ranging from 3 015 to 10 632 Da with a high quality score of 7.38 ± 1.26. Profiles were reproducible over successive experiments performed at 1, 2, and 3‐week growth durations and unambiguously distinguished the Archaea from all of the 3 995 bacterial spectra in the Brüker database. After the incorporation of the determined profiles into a local database, archaeal isolates were blindly identified within 10 min with an identification score of 1.9–2.3. The MALDI‐TOF‐MS‐based clustering of these archaeal organisms was consistent with their 16S rDNA sequence‐based phylogeny. These data prove that MALDI‐TOF‐MS profiling could be used as a first‐line technique for the identification of human Archaea. In complement to previous reports for animal cells, Bacteria and giant viruses, MALDI‐TOF‐MS therefore appears as a universal method for the identification of living unicellular and multicellular organisms.
Document Type: Research Article
Affiliations: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Aix-Marseille Université, Marseille, France
Publication date: 2012-02-01