Sequence analysis of HIV‐1 isolates from Guinea‐Bissau: selection of vaccine epitopes relevant in both West African and European countries
Abstract:Vinner L, Holmgren B, Jensen KJ, Esbjornsson J, Borggren M, Hentze JL, Karlsson I, Andresen BS, Gram GJ, Kloverpris H, Aaby P, da Silva ZJ, Fenyö E‐M, Fomsgaard A. Sequence analysis of HIV‐1 isolates from Guinea‐Bissau: selection of vaccine epitopes relevant in both West African and European countries. APMIS 2011; 119: 487–97.
For a CD8 epitope‐based vaccine to match different geographic locations, the targeted epitopes for cytotoxic T‐lymphocytes (CTLs) must be present in the local circulating HIV‐1 strains. Secondly, the vaccine epitopes should match the host population HLA types. We characterized two new HIV‐1 isolates from Guinea‐Bissau. Also, we have identified 15 subdominant CD8 epitopes representing common HLA super‐types theoretically covering most HLA alleles in any population. Herein we demonstrate that the selected vaccine epitopes are well conserved and simultaneously present in sequences from West Africa and Denmark. Use of the selected epitopes will likely ensure ≥10 immune targets in the majority of candidates for experimental therapeutic vaccination in both geographic regions. Our results warrant testing of the selected vaccine epitopes in both geographic locations.
Document Type: Research Article
Affiliations: 1: Virus Research and Development, Statens Serum Institut, Copenhagen, Denmark 2: Department of Laboratory Medicine, Lund University, Lund, Sweden 3: Department of Experimental Medical Science, Lund University, Lund, Sweden 4: Bandim Health Project, INDEPTH Network, Bissau, Guinea-Bissau
Publication date: 2011-08-01