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Immune clearance gastric carcinoma cells in ascites by activating caspase-9-induced apoptosis

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Abstract:

Chang-Qing F, Yi L, De-Guang W, Qing-Bin S, Xiang-Min H, Na T, Jian-Hua L. Immune clearance gastric carcinoma cells in ascites by activating caspase-9-induced apoptosis. APMIS 2011; 119: 173–179.

Floating gastric adenocarcinoma cells in ascitic fluid are the main cause of peritoneal dissemination. Activation of apoptosis is an important mechanism by which tumor cells are eliminated by the immune surveillance system. Hence, we examined caspase-9 expression and the apoptosis in gastric adenocarcinoma cells in ascitic fluid using immunohistochemistry, real-time polymerase chain reaction and in situ cell death detection kits, flow cytometry. The results revealed strong expression of caspase-9 in 58.49% (31/53) malignant cells and a relatively weak expression of caspase-9 in 41.51% (22/53) malignant cells. The proportion of apoptotic cells in 31 malignant cases with strong caspase-9 expression (35.14 ± 3.42)% was significantly higher than that in 22 malignant cases with relatively weak caspase-9 expression (17.29 ± 7.62)% or in mesothelial cells (10.76 ± 4.21%; p < 0.05). Kaplan–Meier survival curves demonstrated that the patients with low caspase-9 expression showed significantly shorter survival (p < 0.05) than those with high caspase-9 expression. These findings suggest that immune clearance gastric carcinoma cells in ascites activated by caspase-9 helped to improve the prognosis of patients with gastric cancer.

Keywords: Gastric carcinoma; apoptosis; ascitic; caspase-9; immune clearance

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2010.02707.x

Affiliations: 1: Departments of Pathology 2: Genetics, The First Affiliated Hospital, China Medical University, Shenyang 3: Department of Magnetic Resonance Imaging, Hospital of People’s Liberation Army, Weihai 4: Departments of General Surgery 5: Digestive Medicine, The First Affiliated Hospital, China Medical University, Shenyang, China

Publication date: March 1, 2011

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