Pertussis serology: assessment of IgG anti-PT ELISA for replacement of the CHO cell assay

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Abstract:

Dalby T, Sørensen C, Petersen JW, Krogfelt KA. Pertussis serology: assessment of IgG anti-PT ELISA for replacement of the CHO cell assay. APMIS 2010; 118: 968–72.

Two types of serological assays are commonly used for the assessment of pertussis vaccine-induced antibodies; the Chinese hamster ovary cell (CHO cell) assay and the immunoglobulin G (IgG) anti pertussis toxin (PT) enzyme-linked immunosorbent assay (IgG anti-PT ELISA). Recently, both the techniques have been modified to improve performance with sera with interfering activity (CHO cell assay) or with heat-treated sera (IgG anti-PT ELISA). These two improved techniques were compared by the analysis of 100 individual serum samples from a previous clinical trial and 213 sera from a longitudinal serum collection from 20 Danish adults recently vaccinated with the Danish acellular pertussis vaccine. The comparison showed a significant linear correlation between the results of the two assays with a p-value of <0.0001 for the 100 individual samples. We, therefore, conclude that the improved IgG anti-PT ELISA can be used as a replacement for the often troublesome and time-consuming CHO cell assay for the measurement of vaccine-induced human antibodies to PT.

Keywords: Bordetella pertussis; ELISA; antibody; human

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2010.02664.x

Affiliations: 1: Department of Microbiological Surveillance and Research 2: Quality Control Department 3: Bacterial Vaccine Department, Statens Serum Institut, Copenhagen, Denmark

Publication date: December 1, 2010

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