Skip to main content

Intragraft immunological events associated with EBV DNAemia in liver transplant patients

Buy Article:

$51.00 plus tax (Refund Policy)

Abstract:

Loginov R, Halme L, Arola J, Höckerstedt K, Lautenschlager I. Intragraft immunological events associated with EBV DNAemia in liver transplant patients. APMIS 2010; 118: 888–94.

Epstein–Barr virus (EBV) may cause post-transplant lymphoproliferative disorder, but most EBV infections after liver transplantation (Ltx) are clinically silent reactivations. In this study, we investigated the intragraft immunological events associated with EBV DNAemia. Altogether, 105 adult Ltx patients were monitored for EBV DNAemia. Fourteen (13%) patients developed EBV DNAemia during the first year after transplantation. Liver biopsies obtained associated with EBV DNAemia, without evidence of other herpes or hepatitis viruses or rejection, were available from five patients. The numbers of lymphocytes positive for B-cell marker (CD20), T-cell markers (CD3, CD4 and CD8) and IL-2R, adhesion molecules (ICAM-1, VCAM-1 and ELAM-1) and their ligands [lymphocyte function-associated antigen-1 (LFA-1), very late antigen (VLA-4) and Sialyl Lewis X (sLeX)] were demonstrated in liver biopsies by immunohistochemistry, and zero-biopsies from donor livers were used as controls. EBV DNAemia was associated with increased number of CD20-positive (22 ± 30, p = 0.09) and significantly increased numbers of CD3 (80 ± 16, p = 0.001)-, CD4 (23 ± 8, p = 0.009)- and CD8 (38 ± 8, p = 0.001)-positive lymphocytes in the graft. ICAM-1, but not VCAM-1 or ELAM-1, was strongly expressed and the number of LFA-1-positive cells was significantly increased (48 ± 10, p = 0.0002). Low-level EBV DNAemia was associated with B- and especially T-cell infiltration of the graft, as well as an increase in ICAM-1 and the number of LFA-1-positive cells. However, EBV DNAemia or these immunological events did not have any effect on the liver transplant.

Keywords: EBV DNAemia; Liver transplantation; intragraft immunological events

Document Type: Research Article

DOI: https://doi.org/10.1111/j.1600-0463.2010.02675.x

Affiliations: 1: Transplant Unit Research Laboratory, Division of Transplantation and Liver Surgery, Department of Surgery, Helsinki University Hospital and University of Helsinki, Helsinki 2: Department of Pathology, Helsinki University Hospital and University of Helsinki, Helsinki, Finland

Publication date: 2010-11-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more