If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Human herpesvirus-6 in patients with Crohn’s disease

$48.00 plus tax (Refund Policy)

Download / Buy Article:

Abstract:

Sura R, Gavrilov B, Flamand L, Ablashi D, Cartun R, Colombel J-F, Van Kruiningen HJ. Human herpesvirus-6 in patients with Crohn’s disease. APMIS 2010; 118: 394–400.

Human herpesvirus-6 (HHV-6) infections are usually asymptomatic reactivations in immunocompetent persons, but may be severe in immunocompromised individuals. Although primary HHV-6 infection is mainly associated with roseola infantum, it has also been associated with gastroenteritis, diarrhea, and nausea in children. In this study, we investigated the potential role of HHV-6 in Crohn’s disease (CD). Evidence of HHV-6 infection in CD patients and controls was determined by immunohistochemistry (IHC), polymerase chain reaction (PCR), and quantitative real-time PCR (qPCR). Fifty-one tissue blocks from 23 CD patients and 20 tissue blocks from 20 controls were examined. Quantitativereal-time PCR was used to assess HHV-6 viral loads. IHC, PCR and qPCR indicated the presence of HHV-6 in both CD patients and controls. Immunohistochemistry of tissues revealed an almost equal frequency and distribution of positive cells; however, non-specific immunostaining confounded interpretation. HHV-6 DNA was detected in 52% (12/23) of CD and 55% (11/20) of control patients by PCR and in 69.5% (16/23) of CD cases and 65% (13/20) of controls by qPCR. Mean viral load in intestinal tissues was similar in CD and controls (33.4 and 57.9 copies μg−1 DNA, respectively). Finding equal evidence of HHV-6 in patients and controls by multiple methods suggests that this virus is ubiquitous and probably not a cause of CD.

Keywords: Crohn’s disease; HHV-6, human herpesvirus-6; PCR; immunohistochemistry; quantitative real-time PCR

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2010.02613.x

Affiliations: 1: Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs-Mansfield, CT 2: Centre de Recherche du Centre Hospitalier Universitaire de Quebec and Faculty of Medicine, Laval University, Quebec, Canada 3: HHV-6 Foundation, Santa Barbara, CA 4: Immunopathology, Hartford Hospital, Hartford, CT, USA 5: Department of Hepatogastroenterology, CHU Lille and INSERM U795 Université Lille Nord de France, Lille, France

Publication date: May 1, 2010

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more