Skip to main content

Detection of invasive protein profile of Streptococcus pyogenes M1 isolates from pharyngitis patients

Buy Article:

$43.00 plus tax (Refund Policy)

Hasegawa T, Okamoto A, Kamimura T, Tatsuno I, Hashikawa S-N, Yabutani M, Matsumoto M, Yamada K, Isaka M, Minami M, Ohta M. Detection of invasive protein profile of Streptococcus pyogenes M1 isolates from pharyngitis patients. APMIS 2010; 118: 167–78.

Streptococcal toxic shock syndrome (STSS) is a re-emerging infectious disease in Japan and many other developed countries. Epidemiological studies have revealed that the M1 serotype of Streptococcus pyogenes is the most dominant causative isolate of STSS. Recent characterization of M1 isolates revealed that the mutation of covS, one of the two-component regulatory systems, plays an important role in STSS by altering protein expression. We analyzed the M1 S. pyogenes clinical isolates before or after 1990 in Japan, using two-dimensional gel electrophoresis (2-DE) and pulsed-field gel electrophoresis (PFGE). PFGE profiles were different between the isolates before and after 1990. Markedly different profiles among isolates after 1990 from STSS and pharyngitis patients were detected. Sequence analysis of two-component regulatory systems showed that covS mutations were detected not only in STSS but also in three pharyngitis isolates, in which proteins from the culture supernatant displayed the invasive type. The mutated CovS detected in the pharyngitis isolates had impaired function on the production of streptococcal pyrogenic exotoxin B (SpeB) analyzed by 2-DE. These results suggest that several covS mutations that lead to the malfunction of the CovS protein occurred even in pharyngeal infection.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: 2-DE; CovS; M1; Streptococcus pyogenes; pharyngitis

Document Type: Research Article

Affiliations: 1: Department of Bacteriology, Nagoya City University Graduate School of Medical Sciences, Nagoya 2: Department of Molecular Bacteriology, Nagoya University Graduate School of Medicine, Nagoya 3: Nagoya City Public Health Research Institute, Nagoya 4: Aichi Prefectural Institute of Public Health, Nagoya, Japan

Publication date: 01 March 2010

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more