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A selective broth enrichment combined with real-time nuc-mecA-PCR in the exclusion of MRSA

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Abstract:

Pasanen T, Korkeila M, Mero S, Tarkka E, Piiparinen H, Vuopio-Varkila J, Vaara M, Tissari P. A selective broth enrichment combined with real-time nuc-mecA-PCR in the exclusion of MRSA. APMIS 2010; 118: 74–80.

We analyzed the performance of a selective enrichment broth combined with Taqman-based real-time duplex nuc-mecA-PCR to expedite the screening of methicillin-resistant Staphylococcus aureus (MRSA). We found the broth to be able to select MRSA strains (oxacillin MIC range 4–256 μg/ml) from MSSA strains. A total of 31 MRSA strains were found from 1250 clinical samples screened. The nuc-mecA-PCR was positive from all enrichment broths containing MRSA. From the remaining 1219 samples negative for MRSA on culture/subculture, 138 samples were nuc+/mecA+ in PCR. The sensitivity of the test was 93.5%, specificity 88.6%, positive predictive value 17.3%, and negative predictive value 99.8% as compared to culture. Thus, with this method, the negative MRSA results can be reliably reported within 24–48 h from sampling. The method is a practical additional alternative to those already described for the same purpose.

Keywords: MRSA; nuc-mecA-PCR

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2009.02562.x

Affiliations: 1: Division of Clinical Microbiology, HUSLAB, Helsinki University Hospital, Helsinki 2: Department of Virology, Haartman Institute, University of Helsinki 3: National Public Health Institute, Helsinki, Finland

Publication date: January 1, 2010

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