Skip to main content

Prevalence, phenotypic and genetic characteristics of prolyliminopeptidase-negative Neisseria gonorrhoeae isolates in Sweden during 2000–2007

Buy Article:

$43.00 plus tax (Refund Policy)

Johansson E, Fredlund H, Unemo M. Prevalence, phenotypic and genetic characteristics of prolyliminopeptidase-negative Neisseria gonorrhoeae isolates in Sweden during 2000–2007. APMIS 2009; 117: 900–4.

In Neisseria gonorrhoeae culture diagnostics, species confirmation is commonly performed using commercial biochemical tests relying on prolyliminopeptidase (PIP) activity. It was previously shown that one PIP-negative strain was mainly globally transmitted during 2000–2004. The aims were to investigate the prevalence and phenotypic and genetic characteristics of PIP-negative N.¬†gonorrhoeae isolates in Sweden during 2000–2007. Gonococcal isolates (n = 1230) cultured in Sweden during 2000–2007 were characterized using PIP screening, antibiogram, serovar determination, pip and porB gene sequencing, and N.¬†gonorrhoeae multiantigen sequence typing (NG-MAST). Fifteen (1.2%) of the isolates were PIP-negative. Of those, 13 (87%) were indistinguishable to the previously described globally transmitted strain, i.e. displayed serovar IB-4 (Bpyvut), similar antibiograms, ST210 (n = 10)/ST292 (n = 3) and contained an identical single nucleotide pip gene deletion. Wherever high reliance is placed on PIP activity for N.¬†gonorrhoeae species confirmation, changes in the diagnostic strategies may need to be considered and/or monitoring of the PIP activity is crucial.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: NG-MAST; Neisseria gonorrhoeae; Sweden; pip and porB gene sequencing; prolyliminopeptidase

Document Type: Research Article

Publication date: 2009-12-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more