Skip to main content

Phagocytosis of heat-killed Staphylococcus aureus by eosinophils: comparison with neutrophils

Buy Article:

$51.00 plus tax (Refund Policy)

Hatano Y, Taniuchi S, Masuda M, Tsuji S, Ito T, Hasui M, Kobayashi Y, Kaneko K. Phagocytosis of heat-killed Staphylococcus aureus by eosinophils: comparison with neutrophils. APMIS 2009; 117: 115–23.

Eosinophils are characterized by several functional properties, such as chemotaxis, adhesion, superoxide anion production, and degranulation. In this article, we have studied the role of bacterial ingestion by eosinophils in comparison with that by neutrophils. Eosinophils and neutrophils were purified by using the Percoll gradient method followed by selection with CD16-coated immunomagnetic beads and centrifugation through a Ficoll-Hypaque gradient combined with dextran sedimentation, respectively. Both cells were preincubated with anti-FcRIIa mAb (CD32 mAb), anti-FcRIIIb mAb (CD16 mAb), anti-CR3 (CD11b mAb), or anti-CR1 (CD35 mAb) before being examined for phagocytosis of opsonized heat-killed Staphylococcus aureus (S. aureus). Phagocytosis and production of hydrogen peroxide were simultaneously measured by flow cytometry using S. aureus labeled with propidium iodide and stained with 2′,7′-dichlorofluorescein diacetate. Eosinophils showed significantly lower activity than neutrophils in both phagocytosis and hydrogen peroxide production. Phagocytosis by both cells was decreased by heat-inactivated serum. Phagocytosis by neutrophils was significantly inhibited by CD16 mAb and CD32 mAb, whereas that by eosinophils was only inhibited by CD35 mAb. Whereas the mechanism of phagocytosis by neutrophils was mediated by CD16 and CD32, that of eosinophils was modulated by complement receptor 1 (CD35).
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: Eosinophils; Staphylococcus aureus; complement receptor 1; neutrophils; phagocytosis

Document Type: Research Article

Affiliations: 1: Department of Pediatrics; 2: Clinical Science and Laboratory Medicine, Kansai Medical University, Osaka; and

Publication date: 2009-02-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more