Skip to main content

Enhanced phosphorylation of the epidermal growth factor receptor at the site of tyrosine 992 in esophageal carcinomas

Buy Article:

$51.00 plus tax (Refund Policy)


Miyawaki M, Hijiya N, Tsukamoto Y, Nakada C, Kawahara K, Moriyama M. Enhanced phosphorylation of the epidermal growth factor receptor at the site of tyrosine 992 in esophageal carcinomas. APMIS 2008;116:1097–106.

This study aimed to determine whether epidermal growth factor receptor (EGFR), which has been reported to be frequently overexpressed in esophageal carcinoma cells, is actually activated in the cells. Paraffin-embedded specimens of 39 cases of esophageal carcinoma were analyzed immunohistochemically with anti-EGFR polyclonal antibody (α-EGFR Ab) and also an anti-phospho-EGFR-specific polyclonal antibody (α-p-EGFRTyr992 Ab) that specifically recognizes phosphorylated tyrosine 992 of EGFR. All of the 39 cases were found to express EGFR, but the expression levels were not significantly higher than those in basal cells of the normal esophageal epithelium. In 38 of the 39 cases, α-p-EGFRTyr992 immunoreactivity was evident. Interestingly, the positively stained carcinoma cells were not distributed diffusely, and strongly immunostained cells tended to be localized in areas of severe dysplasia and in microinvasive foci just adjacent to the main invasive carcinoma. However, the deeply invasive front never exhibited positive immunoreactivity. The present findings suggest that phosphorylation of EGFR Tyr992 may play some specific functional role in esophageal carcinomas besides promotion of cell proliferation.

Keywords: EGFR; Esophageal carcinoma; autophosphorylation; tyrosine kinase

Document Type: Original Article


Affiliations: 1: Surgery II 2: Molecular Pathology, Oita University Faculty of Medicine, Oita, Japan

Publication date: December 1, 2008


Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more