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Evolution of human immunodeficiency virus type 1 serotypes in northern Tanzania: a retrospective study

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Abstract:

Nyombi BM, Nkya W, Barongo L, Bjune G, Kristiansen KI, Müller F, Holm-Hansen C. Evolution of human immunodeficiency virus type 1 serotypes in northern Tanzania: a retrospective study: APMIS 2008;116:507–14.

The HIV-1 epidemic in Tanzania is characterized by the circulation of heterogeneous virus subtypes. A retrospective study was conducted to determine the changing pattern of circulating HIV-1 subtypes in northern Tanzania. A peptide-binding enzyme immunoassay (PEIA) was employed to analyse 305 HIV-1 positive serum and plasma samples collected between 1985 and 2005. Samples were serotyped using synthetic peptides representing HIV-1 genotypes A–E derived from consensus gp120 V3 sequences. Plasma samples collected in 2005 were V3 genotyped for comparison with PEIA results. In 1985, serotypes A and D were co-circulating while serotype C was first detected in 1990. In 2001 and 2005, serotype C was the most prevalent, serotype A was stable, and serotype D was declining. PEIA is relatively rapid and simple to perform compared to molecular approaches, and is a valuable epidemiological tool in regions with limited resources. HIV-1 classification into serotypes based on antigenic V3 diversity may be a useful screening tool for the identification of HIV-1 variants with regard to diagnosis, treatment, disease progression and candidate vaccine development.

Keywords: HIV-1; HIV-1 serotypes; PEIA; Tanzania

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2008.00996.x

Affiliations: 1: Kilimanjaro Christian Medical College, Moshi, Tanzania 2: Institute of General Practice and Community Medicine, Faculty of Medicine, University of Oslo, Oslo 3: Institute of Microbiology, Rikshospitalet, University of Oslo, Oslo

Publication date: June 1, 2008

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