If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Analysis of children with Chlamydophila (Chlamydia) pneumoniae and Mycoplasma pneumoniae respiratoryinfections by real-time PCR assay and serological tests

$48.00 plus tax (Refund Policy)

Download / Buy Article:

Abstract:

Otomo S, Yamamura JI, Hayashi E, Nakamura T, Kakinuma H, Nakamoto Y, Takahashi H, Karasawa T. Analysis of children with Chlamydophila (Chlamydia) pneumoniae and Mycoplasma pneumoniae respiratory infections by real-time PCR assay and serological tests. APMIS 2008;116:477–83.

We examined 73 children with respiratory infections for Chlamydophila (Chlamydia) pneumoniae and Mycoplasma pneumoniae using real-time PCR assay and serological tests. C. pneumoniae and M. pneumoniae infections were found in 11 (15.1%) and 6 (8.2%) cases, respectively. The sensitivities and specificities of real-time PCR versus definite diagnosis of acute infection were 63.6% and 100% for C. pneumoniae, and 100% and 100% for M. pneumoniae, respectively. C. pneumoniae PCR-negative results appeared to be due to poor growth of the organism. The sensitivity and specificity of ImmunoCard tests were 33.3% and 82.1%, respectively, indicating that the efficacy of rapid diagnosis was disputable. The present results suggest that real-time PCR is suitable for rapid diagnosis as a first screening test to determine first-line antibacterial agents to be used against these infectious diseases.

Keywords: Chlamydophila (Chamydia) pneumoniae; Mycoplasma pneumoniae; real-time PCR; serological tests

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2008.00973.x

Affiliations: 1: Department of Clinical Laboratory Science, Division of Health Sciences, Kanazawa University Graduate School of Medical Science, Kanazawa 2: Department of Pediatrics, Kanazawa Medical University 3: Department of Central Clinical Laboratories, Kanazawa Medical University Hospital, Ishikawa, Japan

Publication date: June 1, 2008

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more