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Analysis of children with Chlamydophila (Chlamydia) pneumoniae and Mycoplasma pneumoniae respiratoryinfections by real-time PCR assay and serological tests

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Otomo S, Yamamura JI, Hayashi E, Nakamura T, Kakinuma H, Nakamoto Y, Takahashi H, Karasawa T. Analysis of children with Chlamydophila (Chlamydia) pneumoniae and Mycoplasma pneumoniae respiratory infections by real-time PCR assay and serological tests. APMIS 2008;116:477–83.

We examined 73 children with respiratory infections for Chlamydophila (Chlamydia) pneumoniae and Mycoplasma pneumoniae using real-time PCR assay and serological tests. C. pneumoniae and M. pneumoniae infections were found in 11 (15.1%) and 6 (8.2%) cases, respectively. The sensitivities and specificities of real-time PCR versus definite diagnosis of acute infection were 63.6% and 100% for C. pneumoniae, and 100% and 100% for M. pneumoniae, respectively. C. pneumoniae PCR-negative results appeared to be due to poor growth of the organism. The sensitivity and specificity of ImmunoCard tests were 33.3% and 82.1%, respectively, indicating that the efficacy of rapid diagnosis was disputable. The present results suggest that real-time PCR is suitable for rapid diagnosis as a first screening test to determine first-line antibacterial agents to be used against these infectious diseases.

Keywords: Chlamydophila (Chamydia) pneumoniae; Mycoplasma pneumoniae; real-time PCR; serological tests

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2008.00973.x

Affiliations: 1: Department of Clinical Laboratory Science, Division of Health Sciences, Kanazawa University Graduate School of Medical Science, Kanazawa 2: Department of Pediatrics, Kanazawa Medical University 3: Department of Central Clinical Laboratories, Kanazawa Medical University Hospital, Ishikawa, Japan

Publication date: June 1, 2008



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