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A protease inhibitor as a potential therapeutic target in cancer: Conversion of plasminogen activator inhibitor-1 to an inactive latent state can be accelerated by a peptide or an antibody binding to the

α-helix D-β-strand 2A loop

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Abstract:

The serpin plasminogen activator inhibitor-1 (PAI-1) is a specific inhibitor of the plasminogen activators and a potential therapeutic target in cancer. Accordingly, formation of a basis for development of specific PAI-1 inactivating agents is of great interest. One possible inactivation mode for PAI-1 is conversion to the inactive, so-called latent state. We have now screened a phage-displayed peptide library with PAI-1 as bait and isolated a 31-residue cysteine-rich peptide which will be referred to as paionin-4. A recombinant protein consisting of paionin-4 fused to domains 1 and 2 of the phage coat protein g3p caused a 2 – 3 fold increase in the rate of spontaneous inactivation of PAI-1. Paionin-4 bound PAI-1 with a KD in the nM range. Using several biochemical and biophysical methods, we demonstrate that paionin-4-stimulated inactivation consists in an acceleration of conversion to the latent state. As demonstrated by site-directed mutagenesis and competition with other PAI-1 ligands, the binding site for paionin-4 was localized in the loop between α-helix D and â-strand 2A. We also demonstrate that a latency-inducing monoclonal antibody has an overlapping, but not identical binding site, and accelerates latency transition by another mechanism. Our results show that paionin-4 inactivates PAI-1 by a mechanism clearly different from other peptides, small organochemical compounds, or antibodies, whether they cause inactivation by stimulating latency transition or by other mechanisms, and that the loop between α-helix D and â-strand 2A can be a target for PAI-1 inactivation by different types of compounds.

Document Type: Abstract

DOI: http://dx.doi.org/10.1111/j.1600-0463.2008.001165_67.x

Publication date: May 1, 2008

mksg/apm/2008/00000116/00000005/art00076
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