Performance of Bordetella pertussis IS481 real-time PCR in a vaccine trial setting
Abstract:Gullsby K, Hallander HO, Bondeson K. Performance of Bordetella pertussis IS481 real-time PCR in a vaccine trial setting. APMIS 2007;115:1370–5.
A real-time PCR method targeting the Bordetella pertussis IS481 gene fragment was evaluated in a vaccine trial setting in which real-time PCR results could be validated against culture and serology results. Two commonly used DNA extraction methods, Amplicor® Respiratory Preparation kit and the QIAamp® DNA Mini Kit, were compared. An approximately 50-fold higher sensitivity was achieved using the Amplicor kit. 89 of 276 aspirates analysed with the IS481 real-time PCR were positive. Interestingly, six of these were culture negative and came from serology-negative patients. Defining true positive cases either as culture-positive or as PCR-positive cases that had been confirmed with a serology-positive result or verified with a newly constructed recA PCR, the sensitivity and specificity of the IS481 real-time PCR were 89% and 98%, respectively. This study confirms the specificity and high diagnostic sensitivity of IS481-based PCR methods for diagnosis of B. pertussis.
Document Type: Research Article
Publication date: December 1, 2007