Immunohistochemical analysis of phospho-BAD protein and mutational analysis of BAD gene in gastric carcinomas

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Abstract:

Jeong EG, Lee SH, Kim SS, Ahn CH, Yoo NJ, Lee SH. Immunohistochemical analysis of phospho-BAD protein and mutational analysis of BAD gene in gastric carcinomas. APMIS 2007;115:976–81.

Mounting evidence indicates that deregulation of apoptosis contributes to the development of human cancers. BAD, a proapoptotic Bcl-2 family protein, regulates the intrinsic apoptosis pathway. The aim of this study was to explore whether alterations of phospho-BAD (p-BAD) protein that antagonizes apoptosis function of BAD and mutation of BAD gene are characteristics of human gastric cancers. We analyzed expression of p-BAD in 60 gastric adenocarcinomas by immunohistochemistry. Also, we analyzed BAD gene for detection of somatic mutations by single-strand conformation polymorphism (SSCP) assay. p-BAD expression was detected well in normal gastric mucosal epithelial cells, whereas it was detected in only 51% (31 of the 60) of the cancers. There was no somatic mutation of BAD gene in the 60 gastric cancer samples. The decreased expression of p-BAD in malignant gastric epithelial cells compared to normal mucosal epithelial cells suggested that loss of p-BAD expression may play a role in gastric tumorigenesis. The data also suggest that BAD mutation may not be a direct target of inactivation in gastric tumorigenesis.

Keywords: apoptosis; gastric cancer; mutation; phospho-BAD

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2007.apm_804.x

Affiliations: 1: Pathology, 2: Internal Medicine and 3: General Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea

Publication date: August 1, 2007

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