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Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci

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Abstract:

Stepanović S, Vuković D, Hola V, Di Bonaventura G, Djukić S, Ćirković I, Ruzicka F. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS 2007;115:891–9.

The details of all steps involved in the quantification of biofilm formation in microtiter plates are described. The presented protocol incorporates information on assessment of biofilm production by staphylococci, gained both by direct experience as well as by analysis of methods for assaying biofilm production. The obtained results should simplify quantification of biofilm formation in microtiter plates, and make it more reliable and comparable among different laboratories.

Keywords: Biofilm; Staphylococcus; microtiter plate; quantification

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2007.apm_630.x

Affiliations: 1: Department of Bacteriology, Institute of Microbiology and Immunology, School of Medicine, Belgrade, Serbia, 2: Institute for Microbiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic and 3: Laboratory of Clinical Microbiology, Aging Research Center Ce.S.I., “G. D'Annunzio” University of Chieti-Pescara, Chieti, Italy

Publication date: August 1, 2007

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