Aerococcus urinae: polyphasic characterization of the species

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Abstract:

Christensen JJ, Kilian M, Fussing V, Andresen K, Blom J, Korner B, Steigerwalt AG. Aerococcus urinae: polyphasic characterization of the species. APMIS 2005;113:517–25.

A polyphasic characterization of Aerococcus urinae is presented. In this study the intraspecies relationships between 26 strains of varying geographical origin were examined by phenotypic tests, ribotyping and multilocus enzyme electrophoresis. The results demonstrated two main phenotypic patterns that could be distinguished in tests for hydrolysis of aesculin, and acid production from amygdalin and salicin. Strains were either negative (n=19) or positive (n=6) in these tests. One strain had a deviating pattern. Heterogeneity within the 19 pattern I strains was demonstrated especially by phenotypic tests (acid production from ribose, mannitol, sorbitol, sucrose and D-arabitol) and by multilocus enzyme electrophoresis. However, DNA sequence analysis of the 16S rRNA (n=7) and gyrB genes (n=3) from strains representing the two main patterns showed no variation in sequences among strains. Comparison of A. urinae and representatives of related taxa by 16S rDNA sequence analysis showed that the taxon is related to, but distinct from, other Aerococcus spp.

Keywords: Aerococcus urinae; DNA sequence analysis; multilocus enzyme electrophoresis; phenotypic tests; ribotyping; transmission electron microscopy

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2005.apm_183.x

Affiliations: 1: Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark 2: Gastrointestinal Infections, Department of Bacteriology, Mycology and Parasitology, 3: Clinical Microbiology, 4: Department of Virology, Statens Serum Institut, Copenhagen, 5: Emerging Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Publication date: August 1, 2005

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