Skip to main content

Ultrastructure and chromogranin A immunogold labelling of ECL cell carcinoids

Buy Article:

$43.00 plus tax (Refund Policy)

Fossmark R, Martinsen TC, Qvigstad G, Bendheim MØ, Kopstad G, Kashima K, Waldum HL. Ultrastructure and chromogranin A immunogold labelling of ECL cell carcinoids. APMIS 2005;113:506–12.

Poorly differentiated neuroendocrine cells can be difficult to recognise. Sensitive methods are needed to label cells that have lost their ultrastructural features and have reduced concentrations of neuroendocrine markers. In gastric neoplasms, enterochromaffin-like cells might dedifferentiate and lose their characteristic granules and secretory vesicles, making detection of such cells increasingly difficult. However, chromogranin A (CgA) immunogold labelling could provide sensitive and specific detection of gastric neuroendocrine cells. We present ultrastructural findings, CgA immunogold labelling as well as conventional immunohistochemical findings of two human enterochromaffin-like cell carcinoids. Electron-dense granules of poorly differentiated cells were less intensely labelled than granules in well-differentiated cells. Granules with atypical shape as well as punctuate granules previously found in neuroendocrine neoplasms were also CgA labelled. The CgA labelling efficacy after antigen retrieval in an alkaline solution was higher after heating in an autoclave at 135 °C compared to a microwave at 100 °C for both granules and secretory vesicles without significant deterioration of the ultrastructure. In conclusion, the use of CgA immunogold labelling could ensure a specific classification of cells with neuroendocrine granules and be a supplement to immunohistochemical examination of poorly differentiated tumours.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Chromogranin A; ECL cell; carcinoid; immunogold labelling

Document Type: Research Article

Affiliations: 1: Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, 2: Department of Pathology, St. Olav's Hospital, Trondheim, Norway and 3: Department of Pathology, Oita Medical University, Faculty of Medicine, Oita, Japan

Publication date: 01 August 2005

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more