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Effect of monophosphoryl lipid A on antibody response to diphtheria toxin and its subunits

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Abstract:

Caglar K, Aybay C, Ataoglu H. Effect of monophosphoryl lipid A on antibody response to diphtheria toxin and its subunits. APMIS 2005;113:256–63.

Monophosphoryl lipid A (MPL) was evaluated for its ability to enhance the antibody response to diphtheria toxin and its fragment A and fragment B subunits. BALB/c mice were immunized subcutaneously with 1 Lf of diphtheria toxoid in the presence of 25 μg of MPL on days 0 and 14. Two weeks after the second immunization, sera were obtained from the mice and analysed for antibody response to diphtheria toxin and its subunits. A new ELISA method, developed in our laboratory, was used to measure antibody levels against the toxin, fragment A, and fragment B. It was observed that MPL significantly enhanced antibody responses to diphtheria toxin and its subunits. However, there was no statistical difference between anti-A and anti-B responses. The results indicated that MPL seems to be a potential candidate as an adjuvant for future diphtheria vaccine formulation.

Keywords: Diphtheria toxin; fragment A; fragment B; monophosphoryl lipid A

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2005.apm_03.x

Affiliations: 1: Department of Immunology, Faculty of Medicine, Gazi University, and 2: Molecular Biology and Technology Research and Development Unit & Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Ankara University, Ankara, Turkey

Publication date: April 1, 2005

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