Diagnostic PCR: validation and sample preparation are two sides of the same coin

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Abstract:

Hoorfar J, Wolffs P, Rådström P. Diagnostic PCR: validation and sample preparation are two sides of the same coin. APMIS 2004;112:808–14.

Increased use of powerful PCR technology for the routine detection of pathogens has focused attention on the need for international validation and preparation of official non-commercial guidelines. Bacteria of epidemiological importance should be the prime focus, although a “validation infrastructure” once established could easily be adapted for PCR-based detection of viruses and parasites. The aim of standardization should be the widespread adoption of diagnostic PCR for routine pathogen testing. European experience provides the impetus for realization of this vision through preparation of quantitative reference DNA material and reagents, production of stringent protocols and tools for thermal cycler performance testing, uncomplicated sample preparation techniques, and extensive ring trials for assessment of the efficacy of selected matrix/pathogen detection protocols.

Keywords: Diagnostic; PCR; sample preparation; standardization; validation

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2004.apm11211-1207.x

Affiliations: 1: Canadian Research Institute for Food Safety, University of Guelph, Guelph, Ontario, Canada 2: Department of Applied Microbiology, Lund Institute of Technology, Lund University, Lund, Sweden

Publication date: December 1, 2004

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