Rubella virus infection dysregulates the pattern of p63 expression
The effect of rubella virus (RV) on the expression of the p63 isoforms was investigated in Vero cells. The levels of all the TAp63 isoforms were elevated, while the expression of a ∼73 kDa isoform corresponding to ΔNp63α was downregulated in Vero cells infected with the To-336 strain of RV. A ∼66 kDa isoform corresponding to TAp63β was the predominant protein species in RV-infected cells. Semi-quantitative end-point dilution RT-PCR analysis, with TAp63β isoform-specific primers, detected a 4-fold rise in the TAp63β mRNA level following virus infection. Taken together, our data demonstrate that RV infection alters the stoichiometric ratio of the p63 isoforms. The dysregulated pattern of p63 expression observed in RV-infected cells may represent a mechanism whereby RV exerts its pro-apoptotic effect.
Document Type: Research Article
Affiliations: 1: Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary 2: Institute of Medical Microbiology, The National Hospital University of Oslo, Oslo, Norway
Publication date: 2004-10-01