Neutrophil and platelet dynamics at organ level after cardiopulmonary bypass: An in vivo study in neonatal pigs
Abstract:Brix-Christensen V, Rheling M, Flø C, Ravn H, Hjortdal V, Marqversen J, Andersen N, Tønnesen E. Neutrophil and platelet dynamics at organ level after cardiopulmonary bypass. An in vivo study in neonatal pigs. APMIS 2004;112:133–40.
The aim was to investigate if organ dysfunction is a consequence of cell accumulation in the tissue and whether this accumulation is caused by the cardiopulmonary bypass (CPB) procedure.
Twenty-six piglets were used in the sham group (sternotomy, n=12) or in the CPB group (sternotomy, CPB, n=14). Isotope-labeled autologous 99mTc-neutrophils (PMNs) and 111In-platelets were infused and dynamically followed at organ level with a gamma camera before, during, and 4 h after termination of CPB. The CPB group showed a 49% increase in 99m Tc-PMNs in the kidneys in the postoperative period compared to a decrease of 2% in the sham group. A less marked decrease was observed in the lungs and peripheral blood between the two groups. The increased radioactivity at organ level post-CPB could be due to changes in flow, extraction in the organ or accumulation of cells, especially in the kidneys and lungs, and might contribute to temporary organ dysfunction postoperatively.
Document Type: Research Article
Affiliations: 1: Department of Nuclear Medicine, 2: Department of Anaesthesiology, 3: Department of Thoracic and Cardiovascular Surgery, and 4: Department of Biostatistics, Aarhus University Hospital, Denmark
Publication date: February 1, 2004