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Altered immunoglobulin isotype profile and anti-immature worm surface immunoglobulins in mice harboring a praziquantel-resistant Schistosoma mansoni isolate

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Abstract:

Hanallah S, El-Lakkany N, Mahmoud S, Mousa M, Botros S. Altered immunoglobulin isotype profile and anti-immature worm surface immunoglobulins in mice harboring a praziquantel-resistant Schistosoma mansoni isolate. APMIS 2003;111:1125–32.

After placement in mice of PZQ-sensitive and -insensitive S. mansoni isolates obtained from villagers responding and not responding to PZQ, parasitological criteria reflecting their biological development and also the host anti-immature worm immunoglobulin isotypes were examined 8 and 10 weeks post infection. Hepatic granuloma diameter, hepatic histopathological changes and immunolocalization of IgG and IgM on the surface of PZQ-sensitive and -resistant worms were also examined 10 weeks post infection. Data showed that parasitological criteria were not significantly different between mice infected with the PZQ-sensitive and -insensitive S. mansoni isolates. As regards serum immunoglobulins, in mice infected with the PZQ-insensitive S. mansoni isolate, IgG and IgG1 were significantly (p<0.05) lower 8 and 10 weeks post infection, respectively (1.41±0.07 & 1.08±0.10 and 1.35±0.06 & 1.09±0.07) than in mice infected with the PZQ-sensitive S. mansoni isolate (1.73±0.15 & 1.38±0.10 and 1.73±0.17 & 1.54+0.21) after the same observation periods. IgM level was nearly the same while IgE was lower than that recorded in mice infected with the PZQ-sensitive S. mansoni isolate. IgG immunofluorescence was also lower (60%±6.78) on the surface of resistant worms than that of sensitive worms (66.6%±5.27); meanwhile, hepatic granuloma diameter was significantly larger (296.5±3.0 vs 283.6±4.0) in mice infected with the PZQ-insensitive S. mansoni isolate with higher percentage of intact eggs. Differences in the immunogenic make up of PZQ-sensitive and -insensitive S. mansoni isolates qualitatively and/or quantitatively favoring a certain Th cell subpopulation response could be the underlying reason for such differences recorded in the host immunoglobulin isotype response and also the egg-induced hepatic histopathological changes.

Keywords: PZQ-insensitive S. mansoni isolate; immunoglobulins

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0463.2003.apm1111208.x

Affiliations: 1: Immunology, 2: Pharmacology, Theodor Bilharz Research Institute, Guiza, Egypt 3: Parasitology, 4: Pathology and

Publication date: December 1, 2003

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