If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

A multiplex real-time PCR for quantification of HIV-1 DNA and the human albumin gene in CD4+ cells

$48.00 plus tax (Refund Policy)

Download / Buy Article:

Abstract:

Eriksson LE, Leitner T, Wahren B, Boström AC, Falk KI. A multiplex real-time PCR for quantification of HIV-1 DNA and the human albumin gene in CD4+ cells. APMIS 2003;111:625–33.

We have established a simple system for measuring HIV-1 DNA load in CD4+ cells. In a multiplex configuration, a conserved region in the HIV-1 pol gene and a section of the human albumin gene were simultaneously amplified to estimate the number of HIV-1 DNA copies per cellular genome. An established Epstein-Barr virus (EBV) standard system was used to calibrate the HIV-1 quantification. Our multiplex PCR system was tested on different in vitro developed HIV-1 strains and on longitudinal samples from eight patients. The system was able to amplify both in vitro and in vivo samples of various genetic compositions. In all eight patients, HIV-1 DNA was detected and ranged between 0.17 and 51×10−3 copies per CD4+ cell and could be monitored longitudinally, including long-term PI-ART and STI. The measured HIV-1 DNA load may be used to select the best time for the institution or re-institution of therapy.

Keywords: DNA load; HIV-1; PI-ART; RNA load; real-time PCR; therapy interruption

Document Type: Research Article

DOI: http://dx.doi.org/10.1034/j.1600-0463.2003.1110605.x

Affiliations: Department of Virology, Swedish Institute for Infectious Disease Control, Solna,

Publication date: June 1, 2003

Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more