A multiplex real-time PCR for quantification of HIV-1 DNA and the human albumin gene in CD4+ cells

Authors: ERIKSSON, LARS E.; LEITNER, THOMAS; WAHREN, BRITTA; BOSTRÖM, ANN-CHARLOTTE; FALK, KERSTIN I.

Source: Apmis, Volume 111, Number 6, June 2003 , pp. 625-633(9)

Publisher: Wiley-Blackwell

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Abstract:

Eriksson LE, Leitner T, Wahren B, Boström AC, Falk KI. A multiplex real-time PCR for quantification of HIV-1 DNA and the human albumin gene in CD4+ cells. APMIS 2003;111:625–33.

We have established a simple system for measuring HIV-1 DNA load in CD4+ cells. In a multiplex configuration, a conserved region in the HIV-1 pol gene and a section of the human albumin gene were simultaneously amplified to estimate the number of HIV-1 DNA copies per cellular genome. An established Epstein-Barr virus (EBV) standard system was used to calibrate the HIV-1 quantification. Our multiplex PCR system was tested on different in vitro developed HIV-1 strains and on longitudinal samples from eight patients. The system was able to amplify both in vitro and in vivo samples of various genetic compositions. In all eight patients, HIV-1 DNA was detected and ranged between 0.17 and 51×10−3 copies per CD4+ cell and could be monitored longitudinally, including long-term PI-ART and STI. The measured HIV-1 DNA load may be used to select the best time for the institution or re-institution of therapy.

Keywords: DNA load; HIV-1; PI-ART; RNA load; real-time PCR; therapy interruption

Document Type: Research Article

DOI: http://dx.doi.org/10.1034/j.1600-0463.2003.1110605.x

Affiliations: Department of Virology, Swedish Institute for Infectious Disease Control, Solna,

Publication date: June 1, 2003

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