Skip to main content

Influence of endotoxin-induced acute lung injury on pulmonary innate and adaptive immunity

Buy Article:

$51.00 plus tax (Refund Policy)


Holub M, Lawrence DA. Influence of endotoxin-induced acute lung injury on pulmonary innate and adaptive immunity. APMIS 2003;111:571–80.

Objective. The aim of our study was to analyze pulmonary innate and adaptive immune responses during endotoxemia-induced acute lung injury (ALI). Experimental design. Female BALB/c mice were challenged by endotoxin given intraperitoneally and followed for 24 h by unrestrained plethysmographic analysis. After this period, the mice were sacrificed by CO2 anesthesia and lung histopathology, pulmonary and serum levels of pro-inflammatory cytokines, numbers of lymphocyte subsets in blood and lung, and lung-derived macrophages (Mφ) were analyzed. Main results. Animals with endotoxemia demonstrated significant depression of tidal volumes indicating respiratory failure compared to control mice. Lung histopathology of endotoxin-exposed animals revealed alveolar leakage characterizing ALI. Pulmonary levels of interleukin (IL)-1β, IL-6 and interferon (IFN)-γ in animals with endotoxemia were significantly elevated, whereas serum levels of IL-6 only were increased. IFN-γ was strongly expressed by lung-derived Mφ with high CD11b expression, and this subset significantly increased in the lungs after endotoxin challenge. Additionally, the numbers of lung-resident CD4+ and total T-lymphocytes were significantly reduced after challenge. Conclusion. These data suggest that endotoxemia-induced ALI is associated with exaggerated and sustained pulmonary innate immune responses partly mediated by activated Mφ, whereas adaptive immunity in the lungs is compromised.

Keywords: Acute lung injury; CD4+ T lymphocytes; IFN-γ; IL-1β; macrophages

Document Type: Research Article


Affiliations: Clinical and Experimental Endocrinology and Immunology, Wadsworth Center, Albany, USA and

Publication date: May 1, 2003

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Partial Open Access Content
Partial Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more