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Determination of proliferation index in neoplasms using different Ki-67 equivalent antibodies

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Lindboe CF, von der Ohe G. Torp SH. Determination of proliferation index in neoplasms using different Ki-67 equivalent antibodies. APMIS 2003;111:567–70.

Paraffin sections from 23 tumours were immunohistochemically stained with the following four Ki-67 equivalent antibodies: monoclonal MIB-1 (DAKO), monoclonal MM1 (Novocastra), polyclonal NCL-Ki-67p (Novocastra), and polyclonal Rah Ki-67 (DAKO). Ki-67 labelling indices were determined by counting in exactly the same area in each case. MIB-1 showed the highest labelling index in 21 of the 23 cases, and the mean MIB-1 index was approximately 30% higher than that of the other antibodies. The differences between MM1, NCL-Ki-67p and Rah Ki-67 were small and non-significant. There was a positive correlation between each of the four antibodies. As these findings may be of importance when the Ki-67 labelling index is used as a criterion for tumour grading or for clinical prognostication, this necessitate identification of the antibody used in every case.

Keywords: Immunohistochemistry; Ki-67; MIB-1; proliferation index

Document Type: Research Article


Affiliations: Department of Pathology, Department of Laboratory Medicine, Norwegian University of Science and Technology, St. Olav's Hospital, Trondheim, Norway

Publication date: May 1, 2003


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