Skip to main content

Effects of Ocimum gratissimum L. essential oil at subinhibitory concentrations on virulent and multidrug-resistant Shigella strains from Lagos, Nigeria

Buy Article:

The full text article is temporarily unavailable.

We apologise for the inconvenience. Please try again later.

Iwalokun BA, Gbenle GO, Adewole TA, Smith SI, Akinsinde KA, Omonigbehin EO. Effects of Ocimum gratissimum L. essential oil at subinhibitory concentrations on virulent and multidrug-resistant Shigella strains from Lagos, Nigeria. APMIS 2003;111:477–82.

Ocimum gratissimum leaf extracts have been extensively demonstrated to be effective against the various aetiologic agents of diarrhoea, including Shigellae. However, the mechanism of the shigellocidal action of this plant remains to be understood. This study investigated the effects of O. gratissimum essential oil (EO) at subinhibitory concentrations of 0.75 and 1.0 μg/ml on virulence and multidrug-resistant strains of 22 Shigella isolates from Nigeria. Compared with untreated Shigella strains, O. gratissimum EO caused significant decreases (p<0.01) in extracellular protease activity, o-lipopolysaccharide rhamnose content and incidence of invasiveness mediated as keratoconjunctivitis in guinea pig. The disparity in extracellular protease activity and o-lipopolysacharide rhamnose between the two treatment groups was also found to be significant (p<0.05), suggesting greater anti-virulent effects of O. gratissimum oil at 1.0 μg/ml. Antibiotic susceptibility testing revealed that the EO of O. gratissimum reduced the MICs of antibiotics to which Shigellae showed resistance by 9.8–53.1% and fluoroquinolones by 18.2–45.5%. The results of this study strongly suggest inhibition of extracellular protease and expression of O-LPS rhamnose in Shigellae by O. gratissimum EO. The future use of O. gratissimum– antibiotic combinations as a therapeutic measure against shigellosis is discussed.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: Antibiotic susceptibility; Ocimum gratissimum essential oil (EO); Shigellae; mechanism; virulence

Document Type: Research Article

Affiliations: 1: , College of Medicine, University of Lagos, Biochemistry Department 2: , Nigerian Institute of Medical Research (N.I.M.R), Yaba, Lagos and Microbiology and Genetic Department 3: , Nigerian Institute of Medical Research (N.I.M.R), Yaba, Lagos, Nigeria

Publication date: 2003-04-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more