If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Nitric oxide generation from hydroxylamine in the presence of neutrophils and in the cell-free system

The full text article is temporarily unavailable.

We apologise for the inconvenience. Please try again later.

Download / Buy Article:

Abstract:

Conversion of hydroxylamine ( HA) to nitric oxide ( NO) has been studied in the presence or absence of human neutrophils with or without myristate acetate phorbol ( PMA), catalase ( CAT), hydrogen peroxide ( H2O2 ), and superoxide dismutase ( SOD) and nitric oxide synthase (NOS) inhibitors. The generation of NO from HA in the presence of neutrophils was higher than in the cell-free system. We found that catalase did not influence the nitrite generation from HA in the cell-free system and in the presence of neutrophils. The H2O2 enhanced the NO generation from HA in the presence of neutrophils only. When catalase and H2O2 were added together, a high increase of NO generation from HA in both systems was observed. The addition of SOD decreased whereas addition of PMA enhanced the NO generation from HA in the presence of neutrophils. The presented data show the possible role of oxygen radicals in the decomposition of HA to NO. The addition of NOS inhibitors to the culture of neutrophils decreased the generation of nitrite from HA. Our results suggest that NO generation from HA, which is an intermediate in NO production from L-arginine, may be supported by an enzymatic pathway in which cellular NO synthase is involved.

Keywords: Nitric oxide; hydroxylamine; neutrophils

Document Type: Original Article

Affiliations: Microbiology and Virology Center, Polish Academy of Sciences, Lodz, Lodowa, Poland

Publication date: August 1, 2001

Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more