A new method to measure phthalate exposure in humans
Abstract:We developed a new method for phthalate exposure assessment that uses glucuronidase digestion of urine, solid‐phase extraction followed by liquid chromatography tandem mass spectrometry to analyze eight mono‐esters of phthalates (1, 2). The mono‐esters, one of the primary human metabolites of phthalate diesters, are monitored to avoid the ubiquitous background contamination of phthalate diesters. This method is sufficiently sensitive to measure urinary levels in non‐occupationally exposed humans. The technique can also be used to measure the degree of glucuronidation of the phthalate metabolites in urine.
1. Blount BC, Milgram KE, Silva M, Malek N, Reidy JA, Needham LL and Brock JW. Quantitative detection of eight phthalate metabolites in human urine using HPLC‐APCI‐MS/MS. Analyt Chem 2000;72:4127‐34.
2. Blount BC, Silva MJ, Caudill SP, Needham LL, Pirkle JL, Sampson EJ, Lucier GW, Jackson RJ and Brock JW Urinary phthalate metabolites in a reference human population. Environ Health Perspect 2000;108:979‐82.
Document Type: Research Article
Affiliations: National Center for Environmental Health, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA
Publication date: July 1, 2001