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The putative R1 protein of Streptococcus agalactiae as serotype marker and target of protective antibodies

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Abstract:



The streptococcal R1 protein was studied by means of anti-R1 antibodies prepared by appropriate cross-absorption of rabbit antiserum raised against the group B streptococcal (GBS) strain ATCC 12403 (D136C), serotype III/R1. The protein was a ladder-forming antigen according to banding patterns in immunoblotting, similar to several other GBS proteins, and was susceptible to digestion by both pepsin and trypsin. Antibody-based testing revealed that 10% of Norwegian GBS isolates expressed the R1 protein, most frequently capsular antigen type V strains (72%) and less frequently type III strains (3%). None of 132 GBS strains from Zimbabwe, including 39 type V strains, expressed the R1 protein. R1-specific rabbit antibodies showed protective activity in mice challenged with a GBS type V/R1 strain. The results show that the R1 protein is an important GBS serotype marker in strains from certain geographical areas, notably for the subtyping of capsular type V strains, and that this protein is a target of protective antibodies.

Keywords: R1 protein; Streptococcus agalactiae

Document Type: Original Article

Affiliations: 1: Department of Medical Microbiology, University of Zimbabwe Medical School, Harare, Zimbabwe, and 2: Department of Microbiology, The Norwegian University of Science and Technology (NTNU), Trondheim, Norway

Publication date: December 1, 2001

mksg/apm/2001/00000109/00000012/art09206
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