Production and partial characterization of mouse monoclonal antibodies recognizing common cytokine receptor gamma chain (γc) of human, mouse and primate origin

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Monoclonal antibodies specific for the common cytokine receptor gamma chain, γc, were produced using traditional hybridoma technology. Fusion of P3X63-Ag8.653 myeloma cells with splenocytes from Balb/c mice immunized with Spodoptera frugiperda insect cells infected with the recombinant baculovirus VL1392-hIL-2Rγ resulted in several hybridoma cell clones producing monoclonal γc-specific antibodies. Four of these antibody-producing clones, IIIC3, IIIE8, IG3 and IF10C5, were further characterized by immunoblotting, flow cytometry and ELISA. Data are presented demonstrating that the generated monoclonal antibodies can identify the extracellular domain of the common cytokine receptor γ chain of human and mouse origin, and two of the antibodies recognize γc of primate origin as well.

Keywords: IL-2; IL-2R; Monoclonal antibodies; baculovirus; c

Document Type: Original Article

Affiliations: 1: Department of Biology, Åbo Akademi University, Turku and 2: Department of Biochemistry and Pharmacy, Åbo Akademi University, Turku, Finland

Publication date: October 1, 2001

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