Skip to main content

Detection of mRNA for the terminal complement components C5, C6, C8 and C9 in human umbilical vein endothelial cells in vitro

Buy Article:

The full text article is temporarily unavailable.

We apologise for the inconvenience. Please try again later.

Human umbilical vein endothelial cells (HUVEC) have previously been shown to synthesize the functional terminal pathway of complement based on the detection by radioimmunoassay of the terminal complement complex (TCC) on coincubated agarose beads. In addition, C7 secretion by these cells in amounts comparable to C3, as well as C7 mRNA, has recently been demonstrated. However, it has not been possible to detect C5–6 and C8 in the fluid phase, and only trace amounts of soluble C9. Against this background we examined whether mRNA for the remaining terminal complement factors was present in HUVEC. By the use of reverse transcription (RT)-polymerase chain reaction (PCR) and Northern blot the presence of mRNA for complement factors C5, C6, C8 and C9 was demonstrated.

No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: HUVEC; TCC; mRNA

Document Type: Original Article

Affiliations: 1: Research Forum and Department of Gastroenterological Surgery and 2: Department of Medical Genetics, Ullevål University Hospital, Oslo, Norway, 3: Department of Physiology and Pathology, University of Trieste, Italy, 4: Department of Environmental Medicine, National Institute of Public Health, Oslo, Norway and

Publication date: 2001-01-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more