IngentaConnect CD30 expression on circulating memory CD4 T cells as a Th2-domina...

Authors: Yamamoto, J.¹; Adachi, Y.¹; Onoue, Y.¹; Kanegane, H.¹; Miyawaki, T.¹; Toyoda, M.²; Seki, T.²; Morohashi, M.²

Source: Allergy, Volume 55, Number 11, November 2000 , pp. 1011-1018(8)

Publisher: Blackwell Publishing

Abstract:

Th2 clones have been reported to express CD30 preferentially, but whether T cells producing Th2-type cytokines may favor CD30 expression in the in vivo state remains unknown. We investigated the expression of CD30 on circulating T cells in atopic dermatitis (AD) as a Th2-dominated disorder. Peripheral blood mononuclear cells were prepared from 51 AD patients and 14 nonatopic controls, and their phenotypes were analyzed with flow cytometry. Cytokine production by stimulated CD4⁺ T cells was also assessed by the single-cell-staining method. Flow cytometric analysis clearly revealed that CD30⁺ T cells were identifiable in the blood of AD patients with greater frequency compared to controls. The important finding was that CD30 expression was restricted to a small but substantial population of memory (CD45RO⁺) CD4⁺ T cells, but not CD8⁺ ones. In AD patients, it was demonstrated that the percentages of CD30⁺ cells within CD45RO⁺ CD4⁺ T cells correlated well with the disease severity, serum IgE levels, peripheral eosinophil counts, and tendency toward Th2-dominant cytokine pattern as determined by the ratio of interleukin-4 to interferon-gamma production. This study suggests that CD30 expression in circulating T cells might serve as an in vivo marker for the Th2-dominated condition.

Keywords: atopic dermatitis; CD30; human Th1/Th2 cells; interferon-gamma; interleukin-4

Document Type: Research article

DOI: 10.1034/j.1398-9995.2000.00685.x

Affiliations: 1: Department of Pediatrics, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan 2: Department of Dermatology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan

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CD30 expression on circulating memory CD4+ T cells as a Th2-dominated situation in patients with atopic dermatitis

CD30 expression on circulating memory CD4⁺ T cells as a Th2-dominated situation in patients with atopic dermatitis