Immunocontraceptive Effect of DNA Vaccine Targeting Fertilin β in Male Mice

Authors: Sun, Zhongyi; Jin, Fengshuo; Li, Yanfeng; Zhang, Jun

Source: American Journal of Reproductive Immunology, Volume 63, Number 4, April 2010 , pp. 282-290(9)

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Abstract:

Citation Sun Z, Jin F, Li Y, Zhang J. Immunocontraceptive effect of DNA vaccine targeting fertilin β in male mice. Am J Reprod Immunol 2010; 63: 282-290 Problem

In previous study, two eukaryotic expression plasmids pSG.SS.YL-F_β.ECD and pSG.SS.C3d3.YL-F_β.ECD were successfully constructed and transfected in HEK293 cells. Now, we want to evaluate the immunocontraceptive effect of these two DNA vaccines that target the extracellular domain (Fβ._ECD) of sperm antigen fertilin β subunit in Kunming male mice. Method of study

DNA vaccines pSG.SS.YL-F_β.ECD and pSG.SS.C3d3.YL-F_β.ECD were injected into Kunming male mice three times at 0, 4, and 8 weeks, respectively. An antifertility effect was observed. Serum antibody and cytokines were also detected. Results

Both vaccines significantly decreased both the pregnancy rate and the number of newborns. The serum levels of IL-2 and INF-γ significantly decreased, whereas the levels of IL-4 and IL-10 significantly increased. Compared with pSG.SS.YL-F_β.ECD, pSG.SS.C3d3.YL-F_β.ECD was more effective in birth control, and its specific F_β-IgG antibody titer in serum was significantly higher and longer. Conclusion

The results indicate that both pSG.SS.YL-F_β.ECD and pSG.SS.C3d3.YL-F_β.ECD DNA vaccines are effective in birth control of mice. The immunocontraceptive effect of F_β.ECD DNA vaccine in male mice is improved with the addition of immuno-adjuvant C3d3.

Keywords: Antigen; contraception; DNA vaccine; fertilin β; immuno-adjuvant; sperm

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1600-0897.2009.00785.x

Publication date: 2010-04-01

Immunocontraceptive Effect of DNA Vaccine Targeting Fertilin β in Male Mice

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