Home >> American Journal of Reproductive Immunology, Volume 62, Number 1

The Role of IL-6, IL-10, TNF-α and its Receptors TNFR1 and TNFR2 in the Local Regulatory System of Normal and Impaired Human Spermatogenesis

Authors: Białas, Małgorzata1; Fiszer, Dorota1; Rozwadowska, Natalia1; Kosicki, Włodzimierz2; Jedrzejczak, Piotr3; Kurpisz, Maciej1

Source: American Journal of Reproductive Immunology, Volume 62, Number 1, July 2009 , pp. 51-59(9)

Publisher: Wiley-Blackwell

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Abstract:

Problem 

To investigate the expression of genes coding for selected cytokines with antagonistic functions (IL-6, IL-10, TNF-α) as well as TNF-α receptors (TNFR1 and TNFR2) in correct spermatogenesis (normal proliferation), maturation arrest (proliferation inhibited) and testicular tumors (overgrowth). Method of study 

Transcription levels of genes coding for IL-6, IL-10, TNF-α, TNFR1 and TNFR2 were quantitatively examined using a real-time RT-PCR. Results 

Significantly higher amounts of IL-6 mRNA were observed in testicular tumor samples than in normal spermatogenesis or in some syndromes with maturation arrest (MA at spermatid level or SCOS), while IL-10 gene levels were fairly stable. In homogenates with maturation arrest, the expression of TNFR1 gene was markedly higher than in testicular tumors, while the opposite phenomenon was found in respect to TNFR2 gene. Conclusion 

The results obtained indicate that changes in activities of intra-testicular cytokines may promote different distinct pathologies such as testicular cancer or infertility.

Keywords: Azoospermia; cytokines; human testis; testicular tumors

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1600-0897.2009.00711.x

Affiliations: 1: Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland 2: Department of Surgery, State District Hospital, Poznań, Poland 3: Clinic of Infertility and Reproductive Endocrinology, Poznań Medical University, Poznań, Poland

Publication date: 2009-07-01

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