Skip to main content

Differential release of matrix metalloproteinase-9 and nitric oxide following infusion of endotoxin to human volunteers

Buy Article:

$43.00 plus tax (Refund Policy)


Roughly 400 000 cases of sepsis occur every year in the United States only and this is associated with a very high mortality. Bacterial lipopolysaccharide (LPS) triggers systemic inflammatory reactions in sepsis. However, down-stream cellular cascade initiated by LPS is still being elucidated. Nitric oxide (NO) and matrix metalloproteinases-2 and ‐9 (MMP-2 and MMP-9) are known to be induced by LPS. We have investigated the release of NO, MMP-2 and MMP-9 following infusion of LPS to volunteers. Methods:

IPS (2 ng kg−1) was infused to 10 healthy volunteers. Before the experiments were started the subjects had an intravenous catheters placed. An electrocardiogram was also placed and monitored constantly. Body temperature was measured by ear thermometer every 10 min Venous blood was collected and cell-free plasma assayed for the presence of MMP-2 and MMP-9 using zymography and NO using HPLC assay for NO metabolites, nitrite and nitrate. Results:

The administration of LPS resulted in increased body temperature and tachycardia. Time-dependent release of MMP-9(30 fold increase from the baseline) peaking at 2 h following infusion of LPS was observed. LPS did not significantly modify the activity of MMP-2 (P > 0.05). Infusion of LPS did not significantly change the levels of nitrite and nitrate (from 60 ± 11 to 67 ± 10 µm, P > 0.05). Conclusion:

The release of MMP-9, but not MMP-2 or NO, is a sensitive index of endotoxaemia in humans. MMP-9 release may contribute to the pathogenesis of sepsis via its pro-inflammatory effects on the vasculature.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: endotoxin; metalloproteinase; nitric oxide; sepsis

Document Type: Research Article

Affiliations: 1: Karolinska Hospital, and 2: Department of Pharmacology, University of Alberta, Edmonton, Alberta, Canada 3: Huddinge University Hospital, and 4: Paediatric Intensive Care Programme, Karolinska Hospital, Stockholm, Sweden, and

Publication date: 01 April 2003

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more