Adsorption of adenine, cytosine, uracil, ribose, and phosphate by Mg-exchanged montmorillonite
Authors: Hashizume, H.; van der Gaast, S.; Theng, B. K. G.
Source: Clay Minerals, Volume 45, Number 4, December 2010 , pp. 469-475(7)
Publisher: Mineralogical Society
Abstract:We have determined the adsorption of RNA components (adenine, cytosine, uracil, ribose and phosphate) by Mg2+-exchanged montmorillonite. The isotherms for adenine, cytosine and uracil were of the C-type, the amount adsorbed increasing linearly with the equilibrium solute concentration. All three nucleic acid bases were apparently adsorbed by coordination to Mg2+ ions through a water bridge. Very little ribose was adsorbed by Mg-exchanged montmorillonite. The isotherm for phosphate was of the L-type. The plateau value of about 0.012 mmol g−1 indicated that phosphate adsorbed on the edge surface of montmorillonite. At comparable solute concentrations, adsorption decreased in the order adenine > cytosine > uracil. This observation reflects differences in basicity, size, and aqueous solubility among the three compounds.
Document Type: Research Article
Publication date: December 2010